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Endophytes are fungal microorganisms that colonize the tissues of virtually all plants and can exhibit a variety of relationships from mutualism to parasitism with their hosts. Ten endophytes isolated from five northwest coniferous tree species were grown on seven different types of agar media made from three different host species and potato dextrose. Preliminary results suggest that some endophytes grow better on tree media than potato dextrose, and some are more host specific than others.